Figure 3

Histological and immunohistochemical images of canine mammary liposarcoma. The histological sample was stained with the standard hematoxylin and eosin (H-E) method. Immunopositivity (nuclear or cytoplasmic) is shown as brown precipitate in neoplastic cells. The EnVision + System-HRP detection system was used, and the signal was visualized with chromogen 3,3-diaminobenzidine 3-3' (DAB). Arrows indicate positive nuclear staining of cells. The images were obtained under the Olympus BX41 microscope. Original magnification: H-E (x400), Vim (x400), Ki67 (x1000), PR (x400), p53 (x400).