Figure 5

Immunohistochemistry analysis: Immunohistochemistry for collagens type I and II (“Col1 and Col2”), and alpha smooth muscle actin (“ASM”), of hyperconfluent cell sheets (“HCS”) and tensioned synoviocyte bisocaffolds (“TSB”). Immunohistochemistry negative controls are delineated by “NC.” 10X objective magnification, bar = 100 μm; Nova Red chromogen. In this example, TSB has moderate extracellular matrix staining for type I collagen, moderate intracellular immunoreactivity to type II collagen, and strong intracellular immunoreactivity to alpha smooth muscle actin.