Figure 7

Effect of AKT on heat-induced apoptosis. (A) Phosphorylation of AKT and BAD in rat small intestine was significantly increased after heat exposure. (B) AKT and Bad proteins extracted from rat IEC-6 cells. Cells were treated with heat for 15 min, 30 min, 1 h, 2 h, 4 h and 8 h, respectively. The phosphorylation of AKT and BAD were significantly higher at 2 h of heat exposure than at other time points. (C) was observed after heat exposure. Compared to control, heat stress caused damages to cell morphology. The damage was more serious at 4 h (b,d) of heat exposure than at 2 h(a,c)(a,b 200×; c,d 400×). *p <0.05, ** p <0.01 heat-stressed versus control.