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Table 2 Frequency of detection of Brucella spp. 16S rRNA and 31 Kd protein genes DNA and cDNA in different samples of H. tuberculatus collected from seropositive and seronegative buffaloes

From: Detection of Brucella abortus DNA and RNA in different stages of development of the sucking louse Haematopinus tuberculatus

Sample

Frequency (% positive)

 

Seropositive buffaloes

Seronegative buffaloes

 

16S rRNA

31 Kd protein

16S rRNA

31 Kd protein

 

DNA

cDNA

DNA

cDNA

DNA

cDNA

DNA

cDNA

Adults

24/36 (66.7)AB

20/24 (83.3)

8/36 (22.2)

4/8 (50.0)

0/36 (0.0)

NP

0/36 (0.0)

NP

Nymphs

16/36 (44.4)A

13/16 (81.3)

8/36 (22.2)

4/7* (57.1)

0/36 (0.0)

NP

0/36 (0.0)

NP

Nits

16/18 (88.9)B

14/16 (87.5)

8/18 (44.4)

5/8 (62.5)

0/36 (0.0)

NP

0/36 (0.0)

NP

  1. The amount of DNA and cDNA were normalized by using the housekeeping (buffalo β actin gene).
  2. *RNA was not detected in one sample.
  3. NP = Not performed.
  4. Values with different superscripts within columns are significantly different (A,B, P < 0.01).