Table 1 Original real time PCR primers and probes used in this study for 16S RNA, 31 KDa (bcsp31 ) and buffalo β actin genes

16S RNA

5′- GCGCGTAAGGATGCAAACAT -3′

5′- CTTGCCTTTCAGGTCTGC-3′

5′- GGCTCATCCAGCGAAACG -3′

31 KDa

5′- AAACGGTAGGTTGCCTAGAG -3′

5′- AATGCCTTGTAGGTCTTT-3′

5′- TTATCATCCGGTGAAGAC -3′

β actin

5′-CTGGCACCACACCTTCTACAA -3′

5′- GCCTCGGTCAGCAGCA -3′

5′- CCACGCGCAGCTCG -3′