Figure 6

BMI1 and OCT4 mRNA expression in maintained and differentiated CD34^pos/SP-C^pos/CCSP^poscells. (A) Depending on the culture medium conditions (maintenance, AECII or club medium), the relative proportions of SP-C^pos/CCSP^pos, SP-C^pos/CCSP^neg or SP-C^neg/CCSP^pos were determined at the first passage by flow cytometry and reported in the table as the following: 0% for no cells, + for <15%, ++ ≥15% and ≤50%, and +++ for ≥50%. (B) The BMI1, OCT4 and NANOG mRNAs were detected by RT-PCR from the total cell RNAs extracted from the three culture conditions at passage 1 and compared to the ovine GAPDH housekeeping gene. Prog: progenitors.