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Table 2 Gene primer sequences used for qPCR

From: Isolation of equine multipotent mesenchymal stromal cells by enzymatic tissue digestion or explant technique: comparison of cellular properties

Gene

Primer sequence

Amplicon size

GAPDH

F: CCAGAACATCATCCCTGCTT

158

NM_001163856

R: CGTATTTGGCAGCTTTCTCC

Collagen 1A2

F: GAAGATGGTCACCCTGGAAA

177

XM_001492939

R: AGGTTCACCCTTCACACCTG

Scleraxis

F: ACAGAAAGACGGCGATTCGGAGTT

207

NM_001105150

R: AAAGTTCCAGTGGGTCTGGGCAA

  1. List of genes analysed by real-time quantitative PCR. GenBank accession numbers of the sequences used for primer design with Primer3 free online software as well as primer sequences and length of the amplicons in base pairs are shown. The sequences of primers of the gene scleraxis were kindly provided by the Institute of Anatomy, University of Veterinary Medicine, Foundation, Hannover, Germany. (F: forward, R: reverse).
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BMC Veterinary Research

ISSN: 1746-6148

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