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Table 1 Semiquantitative adipogenic differentiation score

From: Isolation of equine multipotent mesenchymal stromal cells by enzymatic tissue digestion or explant technique: comparison of cellular properties

% of differentiated cells among all MSCs in field of view (10× magnification)

Size and arrangement of lipid droplets

0 points

0 – 5%

0 points

No lipid droplets

1 point

>5 – 50%

1 point

Predominantly few isolated and small-sized (< 1/3 of nucleus diameter) lipid droplets

2 points

>50 – 80%

2 points

Predominantly medium-sized (approximately 1/3 of nucleus diameter) lipid droplets, surrounding the nucleus

3 points

>80 - 100%

3 points

Predominantly large-sized (> 1/3 of nucleus diameter) lipid droplets, merging around the nucleus

  1. Two criteria scoring system for semiquantitative evaluation of adipogenic differentiation following the oil red O staining procedure. A maximum score of 6 points was possible, with a maximum of 3 points for each criterion.