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Table 1 Cytotoxicity as measured by lactate dehydrogenase (LDH) released by SJPL cells incubated with A. pleuropneumoniae strain MBHPP147 for a period of up to 96 hours

From: Method to grow Actinobacillus pleuropneumoniaebiofilm on a biotic surface

Incubation time (h) Cytotoxicity (%)*
  Control SJPL cells SJPL cells infected with A. pleuropneumoniae
24 0 0
48 4.93 ± 4.93 14.67 ± 7.55
72 10.60 ± 10.60 8.15 ± 8.15
96 21.05 ± 21.05 22.65 ± 20.65
  1. *The % represents the released LDH/ total cellular LDH and the data are the means ± SEM of three independent experiments with duplicates. No statistical differences were observed between the control cells and the cells infected with A. pleuropneumoniae strain MBHPP147 (two-way ANOVA).