Surveillance of avian influenza virus type A in semi-scavenging ducks in Bangladesh

BMC Veterinary Research

Table 1 Isolation and identification of AIV type A from cloacal swabs during the period of December’2009 to February’2012

Winter season	Division	Wintering sites	Total no. of sample	No. of HA positive samples	No. of NDV positive samples	No. of AIV positive cases in NDV positive samples	RT-PCR positive samples of AIV	Prevalence of AIV (%)	Seasonal prevalence (%)
	Sylhet	Hakaluki Haor	450	278	126	19 (15.05)^*	146	32.44	26.89
Dec. 2009 to Feb. 2010		Tanguar Haor	225	94	52	6 (11.54)	51	22.67
	Dhaka	Jahangirnagar Lake	225	73	37	4 (10.81)	45	20.00
	Sylhet	Hakaluki Haor	300	145	75	7 (9.33)	63	21.00	18.50
Dec. 2010 to Feb. 2011		Tanguar Haor	150	64	39	3 (7.69)	27	18.00
	Dhaka	Jahangirnagar Lake	150	58	23	2 (8.69)	21	14.00
	Sylhet	Hakaluki Haor	300	163	75	5 (6.67)	58	19.33	18.33
Dec. 2011 to Feb. 2012		Tanguar Haor	150	71	37	2 (5.41)	23	15.33
	Dhaka	Jahangirnagar Lake	150	73	41	2 (4.88)	29	19.33
Total			2100	1019	505	50 (9.90%)	463	22.05

A total of 2100 cloacal swab samples from semi-scavenging domestic ducks were tested for virus isolation through chicken embryo inoculation following by Haemagglutination test and identification was confirmed by RT-PCR from HA positive samples (allantoic fluid).
^*Values in the parenthesis indicates per cent positive.

ISSN: 1746-6148