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Table 2 Transcription levels of different genes in digital dermatitis diseased groups (M1, M2, M3, M4) expressed as fold changes compared to the normal group (M0)

From: Digital dermatitis in cattle is associated with an excessive innate immune response triggered by the keratinocytes

Gene

M1(n=5)

M2(n=5)

M3(n=4)

M4(n=6)

CCR3

0.36±0.10

0.56±0.18

0.36±0.02*

0.39±0.10*

IL1B

4.9±2.5

52.3±21.6

37.3±14.9

2.7±1.4

IL4

0.42±0.19

1.14±0.51

0.13±0.07

0.38±0.16

IL5

0.42±0.08

0.44±0.10

0.30±0.07*

0.40±0.06*

IL6

9.9±4.4

44.4±23.6

37.2±19.0

2.8±1.4

IL8

42.8±33.3

328.0±144.4*

190.7±112.7*

10.8±4.8*

IL10

1.60±0.45

2.9±0.7

1.8±0.5

1.02±0.17

IL13

0.68±0.10

0.74±0.15

0.72±0.23

0.50±0.14*

IL17

2.34±0.96

4.80±2.57

1.55±0.76

1.52±0.74

INFG

ND

ND

ND

ND

TGFB

0.83±0.16

0.95±0.16

0.63±0.09

0.68±0.12

TNFA

0.61±0.18

1.36±0.34

1.35±0.39

1.01±0.15

  1. ND: not expressed, too low in the sample.
  2. Data was expressed as (Mean ± SE) of fold changes in diseased groups compared to normal group.
  3. *p-value < 0.05 is considered significant in comparison to the normal (M0) group.
  4. n: number of samples used per group.