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Table 1 Genes used in qRT-PCR work, referring to the GeneBank accession number and primer sequences

From: Digital dermatitis in cattle is associated with an excessive innate immune response triggered by the keratinocytes

Gene

Primer sequence

Accession number

Ta (°C)

CCR3

F: TGTGTCAACCCCGTGATCTA

NM_001194960.1

60

 

R: AGAGTTCCTGCTCCCCTGTT

  

IL1β

F: AAGGCTCTCCACCTCCTCTC

NM_174093.1

60

 

R: TTTGGGGTCTACTTCCTCCA

  

IL4

F: GCGGACTTGACAGGAATCTC

NM_173921.2

64

 

R: TCAGCGTACTTGTGCTCGTC

  

IL5

F: TGGTGGCAGAGACCTTGACA

NM_173922.1

60

 

R: TTCCCATCACCTATCAGCAGAGT

  

IL6

F: TCCTTGCTGCTTTCACACTC

NM_173923.2

60

 

R: CACCCCAGGCAGACTACTTC

  

IL8

F: GTTGCTCTCTTGGCAGCTTT

NM_173925.2

60

 

R: GGTGGAAAGGTGTGGAATGT

  

IL10

F: TGTATCCACTTGCCAACCAG

NM_174088.1

60

 

R: CAGCAGAGACTGGGTCAACA

  

IL13

F: GGTGGCCTCACCTCCCCAAG

NM_174089.1

60

 

R: GATGACACTGCAGTTGGAGATGCTG

  

IL17

F: GGACTCTCCACCGCAATGAG

NM_001008412.1

60

 

R: TGGCCTCCCAGATCACAGA

  

IFNÏ’

F: TTCTTGAATGGCAGCTCTGA

NM_174086.1

60

 

R: TTCTCTTCGGCTTTCTGAGG

  

TGFβ

F: CTGCTGTGTTCGTCAGCTCT

NM_001166068.1

60

 

R: TCCAGGCTCCAGATGTAAGG

  

TNFA

F: GCCCTCTGGTTCAGACACTC

NM_173966.2

60

 

R: AGATGAGGTAAAGCCCGTCA

  

GAPDH

F: GGGTCATCATCTCTGCACCT

NM_001034034.1

60

 

R: GGTCATAAGTCCCTCCACGA

  

RPLP0

F: CTTCATTGTGGGAGCAGACA

NM_001012682.1

60

 

R: GGCAACAGTTTCTCCAGAGC

  
  1. F: forward primer.
  2. R: reverse primer.
  3. Ta: annealing temperature.