Change in mRNA expression of sirtuin 1 and sirtuin 3 in cats fed on high fat diet

BMC Veterinary Research

Table 2 Sequences and kind of primers used for polymerase chain reaction

Primer	Kind	Sequence (5′-3′)	Applications	Position
SIRT1
1	Sense	GAGAGGCAGTTGGAAGATGG	RT-PCR	47
2	Antisense	CTGTTGCTTCCTGTTTCACG	RT-PCR	2275
3	Sense	CAACGGTTTGGAAGACGATGCTG	3′ RACE	2156
4	Antisense	TCTTCCTCCTCTTCGCCCTCGTCGT	5′ RACE	452
5	Sense	CGCCTTGCAATAGACTTCCC	q-PCR	897
6	Antisense	TGAATTTGTGACAGAGAGATGGTTG	q-PCR	1042
SIRT3
7	Sense	AGGACCTAGCTGAGCTGATTCG	RT-PCR	349
8	Antisense	TGTGTGTAGAGCCGCAGAAG	RT-PCR	656
9	Sense	CTATTTCCTCCGCCTGCTCCACGAC	3′ RACE	603
10	Antisense	AGGCCGCTCCTTGGAGACCTGAAGT	5′ RACE	464
11	Sense	TGCTTCTGCGGCTCTACAC	q-PCR	635
12	Antisense	TGTCTCCCCAAAGAACACGA	q-PCR	864
Beta-actin
13	Sense	GCCAACCGTGAGAAGATGACT	q-PCR	353
14	Antisense	CCCAGAGTCCATGACAATACCAG	q-PCR	481

ISSN: 1746-6148