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Table 3 Comparison of differences in Ct values in tissue and cell culture samples from different salmonid species

From: Design and evaluation of a unique RT-qPCR assay for diagnostic quality control assessment that is applicable to pathogen detection in three species of salmonid fish

Samples Type Specie ELF1α ELF1α GIM-2 Δ Ct value Eficiency (%)
Ct Value Ct Value
ASK-1 Cell culture S. salar 28.134 ± 0.149 25.381 ± 0.114 2.753 96.65
SHK-1 Cell culture S. salar 27.198 ± 0.204 24.237 ± 0.078 2.961 92.78
RTS11 Cell culture O. mykiss 30.384 ± 0.338 25.028 ± 0.191 5.356 96.52
CHSE-214 Cell culture O. tshawytscha 32.768 ± 0.421 24.354 ± 0.262 8.414 93.64
GIMCP Tissue S. salar 29.235 ± 0.107 25.297 ± 0.205 3.938 96.90
GIM017 Tissue O. mykiss 33.467 ± 0.63 26.194 ± 0.199 5.273 96.16
GIM025 Tissue O. kisutch 29.447 ± 0.177 24.693 ± 0.146 4.754 101.24
GIM496 Tissue O. tshawytscha 36.494 ± 0.217 25.393 ± 0.161 11.101 97.22
  1. The ΔCt value was calculated (Ct value ELF1α - Ct value ELF1α GIM-2).