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Table 3 Comparison of differences in Ct values in tissue and cell culture samples from different salmonid species

From: Design and evaluation of a unique RT-qPCR assay for diagnostic quality control assessment that is applicable to pathogen detection in three species of salmonid fish

Samples

Type

Specie

ELF1α

ELF1α GIM-2

Δ Ct value

Eficiency (%)

Ct Value

Ct Value

ASK-1

Cell culture

S. salar

28.134 ± 0.149

25.381 ± 0.114

2.753

96.65

SHK-1

Cell culture

S. salar

27.198 ± 0.204

24.237 ± 0.078

2.961

92.78

RTS11

Cell culture

O. mykiss

30.384 ± 0.338

25.028 ± 0.191

5.356

96.52

CHSE-214

Cell culture

O. tshawytscha

32.768 ± 0.421

24.354 ± 0.262

8.414

93.64

GIMCP

Tissue

S. salar

29.235 ± 0.107

25.297 ± 0.205

3.938

96.90

GIM017

Tissue

O. mykiss

33.467 ± 0.63

26.194 ± 0.199

5.273

96.16

GIM025

Tissue

O. kisutch

29.447 ± 0.177

24.693 ± 0.146

4.754

101.24

GIM496

Tissue

O. tshawytscha

36.494 ± 0.217

25.393 ± 0.161

11.101

97.22

  1. The ΔCt value was calculated (Ct value ELF1α - Ct value ELF1α GIM-2).