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Table 2 Comparative performance of the assays in different species of salmonid tested

From: Design and evaluation of a unique RT-qPCR assay for diagnostic quality control assessment that is applicable to pathogen detection in three species of salmonid fish

 

ELF1 α

ELF1α GIM-1

ELF1α GIM-2

 

Ct value

ΔRn

Ct value

ΔRn

Ct value

ΔRn

O. mykiss

32.31 ± 0.88

0.201 ± 0.039

26.49 ± 0.25

0.811 ± 0.019

21.43 ± 0.080

1.498 ± 0.017

O. kisutch

31.10 ± 0.36

0.238 ± 0.021

27.86 ± 0.17

0.686 ± 0.043

22.71 ± 0.109

1.353 ± 0.019

S. salar

28.55 ± 0.43

0.307 ± 0.021

29.18 ± 0,14

0.519 ± 0.008

23.56 ± 0.017

1.232 ± 0.001

  1. The comparison was made based on the Ct values and relative fluorescence intensities (ΔRn). The probes used in this comparison were depleted of either MGB or LNA. The data are shown as a Ct value ± standard deviation and ΔRn ± standard deviation (n = 3).
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BMC Veterinary Research

ISSN: 1746-6148

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