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Table 2 Comparative performance of the assays in different species of salmonid tested

From: Design and evaluation of a unique RT-qPCR assay for diagnostic quality control assessment that is applicable to pathogen detection in three species of salmonid fish

  ELF1 α ELF1α GIM-1 ELF1α GIM-2
  Ct value ΔRn Ct value ΔRn Ct value ΔRn
O. mykiss 32.31 ± 0.88 0.201 ± 0.039 26.49 ± 0.25 0.811 ± 0.019 21.43 ± 0.080 1.498 ± 0.017
O. kisutch 31.10 ± 0.36 0.238 ± 0.021 27.86 ± 0.17 0.686 ± 0.043 22.71 ± 0.109 1.353 ± 0.019
S. salar 28.55 ± 0.43 0.307 ± 0.021 29.18 ± 0,14 0.519 ± 0.008 23.56 ± 0.017 1.232 ± 0.001
  1. The comparison was made based on the Ct values and relative fluorescence intensities (ΔRn). The probes used in this comparison were depleted of either MGB or LNA. The data are shown as a Ct value ± standard deviation and ΔRn ± standard deviation (n = 3).