Table 1 Alternative assays used to compare efficiency and transversality of detection
Assay N° | Assay name | Name | Sequence (5′ - 3′) | Reference |
|---|---|---|---|---|
1 | ELF1α For | CCCCTCCAGGACGTTTACAAA | [14] | |
1 | ELF1α | ELF1α Rev | CACACGGCCCACAGGTACA | [14] |
1 | FAM-ELF1α | FAM- ATCGGCGGTATTGGAAC | [14] | |
2 | ELF1α GIM-1-For | CCCCTCCAGGAYGTYTACAAA | This work | |
2 | ELF1α GIM-1 | ELF1α GIM-1 Rev | CACACGGCCCACRGGTACW | This work |
2 | FAM- ELF1α GIM | FAM-ATCGGYGGTAT + T + G + G + A + AC-BHQ | This work | |
3 | ELF1α GIM-2 For | GCCCCTCCAGGAYGTYTACAA | This work | |
3 | ELF1α GIM-2 | ELF α GIM-2 Rev | CCACACGGCCCACRGGTAC | This work |
3 | FAM-ELF1α GIM | FAM-ATCGGYGGTAT + T + G + G + A + AC-BHQ | This work |