Comparison of viremia of type II porcine reproductive and respiratory syndrome virus in naturally infected pigs by zip nucleic acid probe-based real-time PCR

Table 5 Primers and ZNA probe used in the multiplex real-time PCR assay for the M gene

Primer or probe	Sequence (5′-3′)	Primer length (bp)	Amplicon length (bp)	Position^a
PRRSV-M177F	CATTCTGGCCCCTGCCCA	18	177	14698-14715
PRRSV-M177R	ACCACTCCYYGYTTDACAGCT	21	177	14874-14854
NA Probe	FAM-CTCGTGTTGGGTGGCAGA-ZNA4 BHQ1	18		14834-14851

^aNucleotide numbering is based on the PRRSV isolate of strain VR-2332 (accession no. DQ176021).

ISSN: 1746-6148