Figure 2

γH2AX staining patterns in equine tendon fibroblasts. (A) A fibroblast from the post-mortem (PM) group, in which little damage was noted. A few large γH2AX puncta (green; arrow) are considered an indicator of normal, reparable DNA damage that occurs during the S-phase transition. Numerous micropuncta (green) seen here in the remainder of the nucleus are not thought to be DNA damage-associated. DAPI nuclear counter-stain (blue). Scale bar = 10 μm (B) Fibroblasts from the abattoir group, containing remarkable numbers of γH2AX puncta (green) indicating severe DNA damage. Micropuncta can still be seen in the background. DAPI nuclear counter-stain (blue). Scale bar = 10 μm (C) Diffuse γH2AX labelling pattern (green) in mitotic fibroblasts, with labelling of the spindle poles and microtubules in the cell on the left. Scale bar = 10 μm.