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Table 2 Abundance of proteins in vaccine A shared with MDBK

From: Bovine neonatal pancytopenia - Comparative proteomic characterization of two BVD vaccines and the producer cell surface proteome (MDBK)

No.

Identified Protein

Accession Number

MW (kDa)

total spectra (%)

unique peptide count

total spectra count

Coverage (%)

1

Complement C4

ENSBTAP00000009019

102

0.150

36

81

30.0

2

Actin, cytoplasmic 2, N-terminally processed

ENSBTAP00000008132

42

0.120

10

64

45.0

3

Pyruvate kinase

ENSBTAP00000044619

61

0.066

17

35

40.0

4

keratin, type II cytoskeletal 8

ENSBTAP00000001108

54

0.057

13

30

31.0

5

Histone H2B type 1

ENSBTAP00000024155

25

0.055

4

29

35.0

6

Thrombospondin-1

ENSBTAP00000002600

129

0.043

15

23

17.0

7

Glyceraldehyde-3-phosphate dehydrogenase

ENSBTAP00000037577

36

0.038

8

20

37.0

8

Alpha-1-antiproteinase

ENSBTAP00000004927

46

0.032

2

17

7.2

9

Heat shock cognate 70 kDa protein 8

ENSBTAP00000017497

71

0.032

12

17

29.0

10

ATP synthase subunit beta, mitochondrial

ENSBTAP00000017710

56

0.028

9

15

25.0

11

Alpha-actinin-4

ENSBTAP00000014894

105

0.026

11

14

19.0

12

Alpha-1-acid glycoprotein

ENSBTAP00000022991

23

0.021

5

11

31.0

13

Vitamin D-binding protein

ENSBTAP00000033386

53

0.019

2

10

7.0

14

Adenylate kinase 2, mitochondrial

ENSBTAP00000023406

26

0.017

7

9

42.0

15

40S ribosomal protein S3

ENSBTAP00000003962

27

0.013

3

7

15.0

16

Alpha-enolase

ENSBTAP00000017839

47

0.013

5

7

18.0

17

ATP synthase subunit alpha, mitochondrial

ENSBTAP00000010806

60

0.011

4

6

12.0

18

Endopin 2

ENSBTAP00000011576

47

0.009

3

5

13.0

19

Hemopexin

ENSBTAP00000004635

52

0.009

3

5

12.0

20

Alpha-2-HS-glycoprotein

ENSBTAP00000000673

38

0.008

3

4

17.0

21

Na+/K+-transporting ATPase subunit alpha-1

ENSBTAP00000001646

113

0.008

3

4

3.3

22

40S ribosomal protein S4

ENSBTAP00000052226

30

0.006

3

3

12.0

23

Complement C3

ENSBTAP00000022979

187

0.006

3

3

4.9

24

60S ribosomal protein L7a

ENSBTAP00000015358

30

0.004

2

2

13.0

25

MHC class I heavy chain isoform 1

ENSBTAP00000031126

40

0.004

2

2

6.4

  1. Proteins identified concurrently in vaccine A and on MDBK cell surface by LC-MS/MS are shown in the order of the protein percentage of total spectra, reflecting their abundance in vaccine A, from lowest to highest concentration. Accession number as listed on Ensembl database (http://www.ensembl.org). Proteins listed were identified with a probability score that is significant with p < 0.05 if the confidence score was >30 at a significance threshold for the Mascot result of p ≤ 0.01. total spectra (%): percentage of total spectra assigned to respective protein. unique peptide count: number of unique peptides for the identified protein. total spectra: number of total spectra assigned to the identified protein. coverage (%): percentage of total amino acid sequence covered with the identified peptides.
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BMC Veterinary Research

ISSN: 1746-6148

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