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Table 2 Abundance of proteins in vaccine A shared with MDBK

From: Bovine neonatal pancytopenia - Comparative proteomic characterization of two BVD vaccines and the producer cell surface proteome (MDBK)

No. Identified Protein Accession Number MW (kDa) total spectra (%) unique peptide count total spectra count Coverage (%)
1 Complement C4 ENSBTAP00000009019 102 0.150 36 81 30.0
2 Actin, cytoplasmic 2, N-terminally processed ENSBTAP00000008132 42 0.120 10 64 45.0
3 Pyruvate kinase ENSBTAP00000044619 61 0.066 17 35 40.0
4 keratin, type II cytoskeletal 8 ENSBTAP00000001108 54 0.057 13 30 31.0
5 Histone H2B type 1 ENSBTAP00000024155 25 0.055 4 29 35.0
6 Thrombospondin-1 ENSBTAP00000002600 129 0.043 15 23 17.0
7 Glyceraldehyde-3-phosphate dehydrogenase ENSBTAP00000037577 36 0.038 8 20 37.0
8 Alpha-1-antiproteinase ENSBTAP00000004927 46 0.032 2 17 7.2
9 Heat shock cognate 70 kDa protein 8 ENSBTAP00000017497 71 0.032 12 17 29.0
10 ATP synthase subunit beta, mitochondrial ENSBTAP00000017710 56 0.028 9 15 25.0
11 Alpha-actinin-4 ENSBTAP00000014894 105 0.026 11 14 19.0
12 Alpha-1-acid glycoprotein ENSBTAP00000022991 23 0.021 5 11 31.0
13 Vitamin D-binding protein ENSBTAP00000033386 53 0.019 2 10 7.0
14 Adenylate kinase 2, mitochondrial ENSBTAP00000023406 26 0.017 7 9 42.0
15 40S ribosomal protein S3 ENSBTAP00000003962 27 0.013 3 7 15.0
16 Alpha-enolase ENSBTAP00000017839 47 0.013 5 7 18.0
17 ATP synthase subunit alpha, mitochondrial ENSBTAP00000010806 60 0.011 4 6 12.0
18 Endopin 2 ENSBTAP00000011576 47 0.009 3 5 13.0
19 Hemopexin ENSBTAP00000004635 52 0.009 3 5 12.0
20 Alpha-2-HS-glycoprotein ENSBTAP00000000673 38 0.008 3 4 17.0
21 Na+/K+-transporting ATPase subunit alpha-1 ENSBTAP00000001646 113 0.008 3 4 3.3
22 40S ribosomal protein S4 ENSBTAP00000052226 30 0.006 3 3 12.0
23 Complement C3 ENSBTAP00000022979 187 0.006 3 3 4.9
24 60S ribosomal protein L7a ENSBTAP00000015358 30 0.004 2 2 13.0
25 MHC class I heavy chain isoform 1 ENSBTAP00000031126 40 0.004 2 2 6.4
  1. Proteins identified concurrently in vaccine A and on MDBK cell surface by LC-MS/MS are shown in the order of the protein percentage of total spectra, reflecting their abundance in vaccine A, from lowest to highest concentration. Accession number as listed on Ensembl database (http://www.ensembl.org). Proteins listed were identified with a probability score that is significant with p < 0.05 if the confidence score was >30 at a significance threshold for the Mascot result of p ≤ 0.01. total spectra (%): percentage of total spectra assigned to respective protein. unique peptide count: number of unique peptides for the identified protein. total spectra: number of total spectra assigned to the identified protein. coverage (%): percentage of total amino acid sequence covered with the identified peptides.