Niacin supplementation increases the number of oxidative type I fibers in skeletal muscle of growing pigs

Khan, Muckta; Ringseis, Robert; Mooren, Frank-Christoph; Krüger, Karsten; Most, Erika; Eder, Klaus

doi:10.1186/1746-6148-9-177

BMC Veterinary Research

Table 1 Characteristics and performance data of primers used for qPCR

From: Niacin supplementation increases the number of oxidative type I fibers in skeletal muscle of growing pigs

Gene	Forward primer (3′-5′)	Product length (bp)	NCBI Genbank	Slope	R^2#	Efficiency*
Gene	Reverse primer (5′-3′)		NCBI Genbank	Slope	R^2#	Efficiency*
Reference genes
ATP5G1	CAGTCACCTTGAGCCGGGCGA	94	NM_001025218	−3.42	0.999	1.96
ATP5G1	TAGCGCCCCGGTGGTTTGC	94	NM_001025218	−3.42	0.999	1.96
ACTB	GACATCCGCAAGGACCTCTA	205	XM_003124280	−3.60	0.998	1.89
ACTB	ACATCTGCTGGAAGGTGGAC	205	XM_003124280	−3.60	0.998	1.89
RPS9	GTCGCAAGACTTATGTGACC	325	XM_003356050	−3.64	0.999	1.88
RPS9	AGCTTAAAGACCTGGGTCTG	325	XM_003356050	−3.64	0.999	1.88
Target genes
COX4/1	GTGGAACTGTACCGCCTGAA	257	XM_003355730	−3.44	1.000	1.95
COX4/1	TTGTCGTAGTCCCACTTGGC	257	XM_003355730	−3.44	1.000	1.95
COX6A1	CTCAGCTCGCATGTGGAAGA	139	NM_001190221	−3.34	0.996	1.99
COX6A1	GATGCGAAGATGGGGGTAGG	139	NM_001190221	−3.34	0.996	1.99
CACT/SLC25A20	GCAAAGCCCATTAGCCCTCT	235	XM_003483178	−3.21	0.988	2.05
CACT/SLC25A20	GAGCACATCCTCTGGGTGTT	235	XM_003483178	−3.21	0.988	2.05
PPARGC1A	TAAAGATGCCGCCTCTGACT	168	NM_213963	−3.94	0.993	1.79
PPARGC1A	TGACCGAAGTGCTTGTTCAG	168	NM_213963	−3.94	0.993	1.79
PPARGC1B	AAGTGCGGCTTCGTCACCTA	216	XM_003124093	−3.28	0.998	2.02
PPARGC1B	GCTGTCGAAATCCATGGCTT	216	XM_003124093	−3.28	0.998	2.02
SLC22A5	TGCATTTGGCTACATGCTGC	174	XM_003123912	−3.76	0.995	1.85
SLC22A5	ATGATCACCTCAGCTTCCTG	174	XM_003123912	−3.76	0.995	1.85
SDHA	CTACGCCCCCGTCGCAAAGG	380	DQ402993	−3.24	1.000	2.03
SDHA	AGTTTGCCCCCAGGCGGTTG	380	DQ402993	−3.24	1.000	2.03
MYH2	GGCCCTTTGATGCCAAGACA	188	NM_214136	−3.45	1.000	1.95
MYH2	GGCCATGTCCTCGATCTTGT	188	NM_214136	−3.45	1.000	1.95
MYH4	GTGCCCTGCTGCCATCAATA	363	NM_001123141	−3.53	1.000	1.92
MYH4	TGCGTAACGCTCTTTGAGGT	363	NM_001123141	−3.53	1.000	1.92
MYH7	TGCCAGCTTGAGCCTCTTTC	380	NM_213855	−3.33	0.999	2.00
MYH7	GTAGCGCTCCTTGAGGTTGT	380	NM_213855	−3.33	0.999	2.00
FATP1	GGTTCCAGCCTGTTGAATGT	275	NM_001083931	−3.44	0.990	1.95
FATP1	AACAAAACCTTGGTGCTTGG	275	NM_001083931	−3.44	0.990	1.95
UCP2	AGTGTGAGACCTGACGAAGC	435	NM_214289	−3.64	0.996	1.88
UCP2	GCTTGACGGAGTCGTAGAGG	435	NM_214289	−3.64	0.996	1.88
UCP3	GCCACTTTGTCTCTGCCTTC	219	NM_214049	−3.49	0.998	1.93
UCP3	CAAACATCACCACGTTCCAG	219	NM_214049	−3.49	0.998	1.93

^#Coefficient of determination of the standard curve.
*The efficiency is determined by [10^(−1/-slope].

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ISSN: 1746-6148

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