Tuberculous lesions in wild boar from Asturias. a) Severely enlarged mandibular lymph node from a wild boar showing generalized tuberculosis. b) Typical lesion found in mandibular lymph node in wild boar in northern Spain, consisting of small area of calcified necrosis (arrow) confined to one anatomical region. c) Histological section of a mandibular lymph node. Granuloma “type 2” consisted of necrotic-calcified granulomas, with few calcium deposits in the necrotic core. Haematoxylin-eosin staining, bar = 200 μm. d) Liver. Positive immunostaining for Mycobacterium bovis located in macrophages in the necrotic area. PAP staining, bar = 20 μm.