Development and validation of TaqMan probe based real time PCR assays for the specific detection of genotype A and B small ruminant lentivirus strains

Table 4 Intra-assay performance of the TaqMan probe based real time PCR assays

	Reaction 1			Reaction 2			Reaction 3
Theoretical copy number	Average Ct	Average calculated copy number	CV %	Average Ct	Average calculated copy number	CV %	Average Ct	Average calculated copy number	CV %
MVV assay
10 ⁶	20	853389	8.67%	20.04	1008108	23.29%	19.83	1007657	7.96%
10 ⁵	23.29	105939	9.25%	23.40	102693	9.62%	23.32	97177	9.14%
10 ⁴	26.69	12190	3.14%	26.66	11365	1.43%	26.64	10483	7.21%
10 ³	30.29	1248	6.60%	30.07	1136	5.75%	30.16	990	7.14%
10 ²	34.65	83	37.84%	34.01	79	1.35%	33.59	103	34.05%
CAEV assay
10 ⁵	21.22	87689	38.76	20.96	94514	31.38	21.71	92044	30.09
10 ⁴	23.55	17265	18.81	24.41	11326	17.53	24.91	11230	16.01
10 ³	27.33	1463	13.65	28.16	1160	20.64	27.94	1193	3.18
10 ²	31.15	66	39.76	32.58	79	17.85	33.06	55	1.84
10	34.4	11	46.69	35.73	13	58.26	35.56	11	2.3

ISSN: 1746-6148