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Table 1 Primers and probes designed in this study

From: Development and validation of TaqMan probe based real time PCR assays for the specific detection of genotype A and B small ruminant lentivirus strains

Primers and probes

Sequence (5′-3′)

Positions in genome

Amplicon length (bp)

 

MVV assay

  

MVVMA F1

GGATACCCCGAGCTCAAAG

520-538*1

101

MVVMA R3

TTYAAKGCCCAYAGACARTT

601-620*1

MVV MA

5' FAM-TCTGTCAAGGTCTCCTTCCCG-3' TAMRA

576-596*1

 

CAEV assay

  

CAEVMA F

GGGAAAAGGGATTATCCTGAG

554-574*2

113

CAEVMA R

GTTTTAAGGCACCAYAAACAATTTC

642-666*2

CAEV MA

5' FAM-TCTGTCAAGTKCTCCCCTCTG-3′TAMRA

619-639*2

 
  1. *1 Numbering according to nucleotide sequence of reference SRLV strain MVV KV1514 [GenBank accession number: M10608].
  2. *2 Numbering according to nucleotide sequence of reference SRLV strain CAEV Co [GenBank accession number: M33677].
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BMC Veterinary Research

ISSN: 1746-6148

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