Figure 2

Characterization of cultured primary ECs and EC lines. Representative examples of immunofluorescence stainings of endothelial cell specific markers. Primary ECs (A and C) demonstrate an intense perinuclear granular immunofluorescence for vWF, whereas the transformed counterparts (B and D) completely lack vWF expression. Both non-transformed and transformed ECs (E-H) react with UEA-1 lectins, as a membrane-associated staining pattern was evident. Primary (I and K) and immortalized (J and L) ECs incorporate DiI-Ac-LDL to the same extent, resulting in an intense punctuate fluorescence with predominantly a perinuclear distribution.