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Table 1 Summary of specificities and sensitivities of three protein subunits

From: Expression and characterization of highly antigenic domains of chicken anemia virus viral VP2 and VP3 subunit proteins in a recombinant E. colifor sero-diagnostic applications

Indirect ELISA to viral protein subunits Cut-off with mean + 2 S.D. Cut 0ff with mean + 3 S.D.
OD value Specificitya(%) Sensitivityb(%) OD value Specificitya(%) Sensitivityb(%)
VP2-396N 0.200 91.7 (11/12) 100 (40/40) 0.236 100 (12/12) 97.5 (39/40)
VP2-345N 0.185 91.7 (11/12) 100 (40/40) 0.217 100 (12/12) 97.5 (39/40)
VP3-246M 0.195 91.7 (11/12) 90 (36/40) 0.232 100 (12/12) 85 (34/40)
  1. a: The percentage of specificity was determined from the numbers of OD405 value below cut-off values in the total ELISA results with 12 negative CAV-infected sera.
  2. b: The percentage of sensitivity was determined from the numbers of OD405 value higher cut-off values in the total ELISA results with 40 positive CAV-infected sera.