Expression and characterization of highly antigenic domains of chicken anemia virus viral VP2 and VP3 subunit proteins in a recombinant E. colifor sero-diagnostic applications

Table 1 Summary of specificities and sensitivities of three protein subunits

Indirect ELISA to viral protein subunits	Cut-off with mean + 2 S.D.			Cut 0ff with mean + 3 S.D.
Indirect ELISA to viral protein subunits	OD value	Specificity^a(%)	Sensitivity^b(%)	OD value	Specificity^a(%)	Sensitivity^b(%)
VP2-396N	0.200	91.7 (11/12)	100 (40/40)	0.236	100 (12/12)	97.5 (39/40)
VP2-345N	0.185	91.7 (11/12)	100 (40/40)	0.217	100 (12/12)	97.5 (39/40)
VP3-246M	0.195	91.7 (11/12)	90 (36/40)	0.232	100 (12/12)	85 (34/40)

^a: The percentage of specificity was determined from the numbers of OD₄₀₅ value below cut-off values in the total ELISA results with 12 negative CAV-infected sera.
^b: The percentage of sensitivity was determined from the numbers of OD₄₀₅ value higher cut-off values in the total ELISA results with 40 positive CAV-infected sera.

ISSN: 1746-6148