Figure 5
The indirect ELISA results obtained using the various recombinant subunit proteins. The five purified GST-fused VP2 subunits (VP2-435N, VP2-345N, VP2-396N, VP2-171C and VP2-318C) and the three VP3 subunit proteins (VP3-123N, VP3-246M and VP3-366C) were reacted with sera from 20 CAV-positive chicken. The mean of the optical density values at 405 nm (OD405) per subunit-base ELISA were determined from experimental triplicates, and the error bars indicate the standard errors of the means. The statistical significant were calculated by Scheffe’s S method which compared the OD405 value of VP2 or VP3 protein subunits to the value of GST recombinant protein. ***: p<0.001; **: p<0.01.