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Figure 4 | BMC Veterinary Research

Figure 4

From: Expression and characterization of highly antigenic domains of chicken anemia virus viral VP2 and VP3 subunit proteins in a recombinant E. colifor sero-diagnostic applications

Figure 4

The expression and purification results for the five recombinant VP2 and three recombinant VP3 subunit proteins. All subunit proteins were purified by GST affinity chromatography after expression in E. coli BL21 (DE3). The presence of the protein was detected by SDS-PAGE (a) and by Western-blotting (b). Anti-GST tag monoclonal antibody was used to recognize the VP2 and VP3 subunit proteins when the various different expression plasmids, as described in FigureĀ 1, were used. Lane M, pre-stained protein marker; lane 1, 4, 7, 10, 13, 16, 19 and 22, before IPTG induction; lane 2, 5, 8, 11, 14, 17, 20 and 23, after IPTG induction and 4 h cultivation; lane 3, 6, 9, 12, 15, 18, 21 and, 24 after purification by GST affinity chromatography. The solid triangles pinpoint the expressed and purified VP2 and VP3 subunit proteins, respectively.

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