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Figure 2 | BMC Veterinary Research

Figure 2

From: Expression and characterization of highly antigenic domains of chicken anemia virus viral VP2 and VP3 subunit proteins in a recombinant E. colifor sero-diagnostic applications

Figure 2

The expression and purification of recombinant CAV viral proteins. The three expression plasmids showed in FigureĀ 1 were express in E. coli BL21 (DE3) and purified by GST affinity chromatography. The recombinant GST-tag proteins were separated by SDS-PAGE (a) and detected by Western-blotting (b). Anti-GST tag monoclonal antibody was used to recognize the recombinant viral proteins. Lane M, pre-stained protein marker; lane BI, before IPTG induction; lane AI, after IPTG induction and cultivation for 4 hr; lane P, purified recombinant GST-tag protein. The red solid triangles pinpoint the various proteins.

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