Figure 3

OVA-specific cytokine production by splenocytes from lambs gavaged with OVA then i.p. immunized with OVA at 4 weeks of age. Lambs (n = 4/group) were gavaged and i.p. immunized as described in Figure 1A. Lymphocyte proliferation (A) and IFN-γ (B) production from ex vivo re-stimulated splenocytes was measured by ELISA 3 weeks post i.p. immunization. Each data point represents an individual animal and median values are indicated by horizontal lines. *p < 0.05.