Skip to main content

Table 1 Primer-specific sequences, annealing temperatures and base pair length for housekeeping genes, cytokines, acute-phase proteins and heat shock protein

From: Evaluation of immune and stress status in harbour porpoises (Phocoena phocoena): can hormones and mRNA expression levels serve as indicators to assess stress?

Cellmediators/mol. biomarkers   Primer sequences (5′–3′) Annealing temperature Base pair length
YWHAZ S AGA CGG AAG GTG CTG AGA AA 58°C 208
  AS TCA TVA CCA GCA GCA ACT TC   
GAPDH S GCC AAA AGG GTC ATC ATC TC 57°C 201
  AS GGG GCC ATC CAC AGT CTT CT   
IL-10 S CCT GGG TTG CCA AGC CCT GTC 62°C 208
  AS ATG CGC TCT TCA CCT GCT CC   
TNFα S GGC TGA ACA CAT ATG CCA AC 57°C 111
  AS TGA AGA GGA CCT GGG AGT AGA   
HP S CTG GCA GGC TAA GAT GGT TT 54°C 75
  AS GTC AGC AGC CAT TGT TCA TT   
CRP S TTC TCG TAT GCC ACC AAG AG 54°C 192
  AS TTC AGA CCC ACC CAC TGT AA   
HSP 70 S GGG GCT GAA CGT GCT GAG G 62°C 280
  AS CCG CTT GTT CTG GCT GAT GTC