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Table 3 PCR and in situ hybridization results for microsporidia in a second set of shrimp ponds

From: The microsporidian Enterocytozoon hepatopenaei is not the cause of white feces syndrome in whiteleg shrimp Penaeus (Litopenaeus) vannamei

Normal shrimp Pond 3 CV5 (n=10) In situ Pond 7 YOT4 (n =5) In situ
sample number 1stPCR 2ndPCR Hyb 1stPCR 2ndPCR Hyb
1 - + - - - -
2 - - + - - -
3 - + + - - ND
4 + + ++ - - ND
5 - - - - - ND
6 - + -    
7 - + +    
8 - - +    
9 - + +    
10 - + +    
Summary Infected 9/10   Infected 0/5  
WFS shrimp Pond 13 BAP (n=10) In situ Pond 6 YOT (n=10) In situ
sample number 1 st PCR 2 nd PCR Hyb 1 st PCR 2 nd PCR Hyb
1* + + + + + ++
2 - + - + + ++
3* - - - + + ND
4 - - - - + ++
5 - - - + + ++
6 - - ND + + ND
7* - - ND - + ++
8* - - ND + + ND
9 - + - - - ND
10 - + - - - ND
Summary Infected 4/10   Infected 8/10  
  1. * Histology of shrimp in these ponds showed no signs of microsporidian infection but 4 showed signs of severe bacterial infections of the HP that may have masked any microsporidians present. The remaining 6 samples looked normal with no signs of microsporidian infection.
  2. Detection of microsporidia by PCR and in situ hybridization in 2 normal and 2 WFS ponds. Hyb ++ indicates extensive positive in situ hybridization reactions in HP tissue while Hyb + indicates light focal positive reactions and Hyb – indicates no reaction. ND Not done.