Table 1 Primer sequences used
| Primer name | Sequence (5′→ 3′) | Size of amplicon (bp) |
|---|---|---|
| MF1 | CCG GAG AGG GAG CCT GAGA | 951 |
| MR1 | GAC GGG CGG TGT GTA CAA A | |
| 1 st PCR step | 779 | |
| ENF779 | CAG CAG GCG CGA AAA TTG TCC A | |
| ENR779 | AAG AGA TAT TGT ATT GCG CTT GCT G | |
| Nested PCR step | 176 | |
| ENF176 | CAA CGC GGG AAA ACT TAC CA | |
| ENR176 | ACC TGT TAT TGC CTT CTC CCT CC | |
| DIG microsporidian probe | 411 | |
| ENF411 | AGG TGG TGT TAA AAG CCA TTG AG | |
| ENR176 | TAC CTC ATG TCT CCA ATC TAC GAT A | |
| DIG GFP probe | 513 | |
| GFPF513 | TTC ATC TGC ACC ACC GGC AAC CTG | |
| GFPR513 | CTG GTA GTG GTC GGC GAG CTG CAC |