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Figure 2 | BMC Veterinary Research

Figure 2

From: Rapid assessment of bovine spongiform encephalopathy prion inactivation by heat treatment in yellow grease produced in the industrial manufacturing process of meat and bone meals

Figure 2

Quantitative analysis of bovine spongiform encephalopathy (BSE) PrPScin heat-treated samples. Homogenates (10%) of BSE-infected spinal cords treated in yellow grease at 140–180°C for 1 or 3 h were diluted with the PrPC substrate and amplified by serial dextran sulfate-protein misfolding cyclic amplification. The dilution ratios examined in each sample are indicated. Quadruplicate samples were analyzed after each round (R1–R4) of amplification by western blotting after digestion with proteinase K. The lanes labeled “N” are samples in which only the PrPC substrate was treated in the same manner. Horizontal lines indicate the positions of molecular-weight markers corresponding to 37, 25, 20, and 15 kDa.

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