Table 1 Sequence of primers used for Q-PCR analysis
Gene | Sense | Antisense | Accession number |
|---|---|---|---|
GAPDH | 5′ CCTTCATTGACCTTCACTACATGGTCTA | 5′ TGGAAGATGGTGATGGCCTTTCCATTG | NM 001034034.2 |
βACTIN | 5′CGCACCACTGGCATTGTCAT | 5′TCCAAGGCGACGTAGCAGAG | K00622.1 |
CD34 | 5′TGGGCATCGAGGACATCTCT | 5′GATCAAGATGGCCAGCAGGAT | AB021662 |
CD45 | 5′CCTGGACACCACCTCAAAGCT | 5′TCCGTCCTGGGTTTTATCCTG | NM 001206523 |
CD73 | 5′TGGTCCAGGCCTATGCTTTTG | 5′GGGATGCTGCTGTTGAGAAGAA | BC114093 |
CD90 | 5′CAGAATACAGCTCCCGAACCAA | CACGTGTAGATCCCCTCATCCTT | BC104530 |
CD105 | 5′CGGACAGTGACCGTGAAGTTG | 5′TGTTGTGGTTGGCCTCGATTA | NM 001076397 |
OC | 5′ TGACAGACACACCATGAGAACCC | 5′ AGCTCTAGACTGGGCCGTAGAAG | EF673278.1 |
CD73 | 5′AATGGCACGATTACCTGGGA | 5′GGGAGGATCACCTTGTACAC | BT026240.1 |
ACAN | 5′ CACTGTTACCGCCACTTCCC | 5′ GACATCGTTCCACTCGCCCT | NM 173981.2 |
COL2A1 | 5′ ATCCATTGCAAACCCAAAGG | 5′ GACATCGTTCCACTCGCCCT | NM 001113224.1 |
SOX9 | 5′CATGAAGATGACCGACGAG | 5′ CGTCTTCTCCGTGTCGGA | AF278703.1 |
AP2 | 5′ CTGGCATGGCCAAACCCA | 5′ GTACTTGTACCAGAGCACC | NM 174314.2 |
PPARγ | 5′ CGCACTGGAATTAGATGACAGC | 5′ CACAATCTGTCTGAGGTCTGTC | BC116098.1 |
NANOG | 5′ GTGTTTGGTGAACTCTCCTG | 5′ GGGAATTGAAATACTTGACAG | NM 001025344.1 |
OCT4 (1) | 5′ ACACTCGGACCACGTCTTTC | 5′ CGCATGGGTACCAGTGCACCTT | AF022987 |
OCT4 (2) | 5′ GTTCTCTTTGGAAAGGTGTTC | 5′ ACACTCGGACCACGTCTTTC | AY490804.1 |