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Table 4 Set up of the test system for the antiviral assay

From: Phytochemical analysis and in-vitro anti-African swine fever virus activity of extracts and fractions of Ancistrocladus uncinatus, Hutch and Dalziel (Ancistrocladaceae)

 

1

2

3

4

5

6

7

8

9

10

11

12

mg/ml

ASF NIG/99 (+)

Acetone (C)

Hexane(C)

DCM (C)

Chloroform (C)

Methanol (C)

Hexane (F)

Methanol (F)

Ethyl acetate (F)

Chloroform (F)

Blank

Placebo (−)

1

+

*

*

*

*

*

*

*

*

*

 

-

0.5

+

*

*

*

*

*

*

*

*

*

 

-

0.25

+

*

*

*

*

*

*

*

*

*

 

-

0.125

+

*

*

*

*

*

*

*

*

*

 

-

0.0625

+

*

*

*

*

*

*

*

*

*

 

-

0.03125

+

*

*

*

*

*

*

*

*

*

 

-

0.015625

+

*

*

*

*

*

*

*

*

*

 

-

0.0078125

+

*

*

*

*

*

*

*

*

*

 

-

  1. (C) = crude extract; (F) = Fraction; DCM = Dichloro Methane; + = Positive control (ASF NIG/99); * = Crude extract or fractions as stated in the table; - = Placebo/Negative control (wash buffer).
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BMC Veterinary Research

ISSN: 1746-6148

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