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Table 3 Observation of activities of Ancistrocladus uncinatus extracts and fractions on haemadsorbing African swine fever virus in-vitro 5-days post infection

From: Phytochemical analysis and in-vitro anti-African swine fever virus activity of extracts and fractions of Ancistrocladus uncinatus, Hutch and Dalziel (Ancistrocladaceae)

  1 2 3 4 5 6 7 8 9 10 11 12
Mg/ml ASF NIG/99 (+) Acetone (C) Hexane(C) DCM (C) Chloroform (C) Methanol (C) Hexane (F) Methanol (F) Ethyl acetate (F) Chloroform (F) Blank Placebo (−)
1 +++ - + - ++ - - - - +   -
0.5 +++ - ++ - ++ - - - - +   -
0.25 +++ - ++ - ++ - - - - ++   -
0.125 +++ - +++ - +++ - - - + ++   -
0.0625 +++ - +++ + +++ - - + + +++   -
0.03125 +++ + +++ ++ +++ ++ + ++ ++ +++   -
0.015625 +++ ++ +++ ++ +++ ++ ++ ++ ++ +++   -
0.0078125 +++ +++ +++ +++ +++ +++ +++ +++ +++ +++   -
  1. +++ = ≥50 rosettes were observed and counted in the well; ++ = 10–50 rosettes were observed and counted; + = ≤10 rosettes were observed and counted. - = no rosette was observed. C = crude extract and F = fraction of extract. N.B. Rosette formation is an indication of virus infection of the macrophage cells in the PBMC medium.
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