Expression and role of PGP, BCRP, MRP1 and MRP3 in multidrug resistance of canine mammary cancer cells

BMC Veterinary Research

Table 2 Primers used for RT-qPCR

Gene symbol	Forward primer	Reverse primer	Optimum annealing temp. (°C)	Optimum annealing time (sec)
pgp	GCTTAACACCCGGCTCACAGAC	TAAGAAAGCGGCACCAATAGAAAT	72	10
bcrp	GAGCTCCTTGTGGTTGAGAA	AGGTGATGGTCATGAGGAGA	72	10
mrp1	TTTACTTTTCCCTCGTGCTG	TATTCAGGGACCAAAGGTCA	72	45
mrp3	AGGATGGACCTGATGACAGA	AACTTGGGAATCAGGAGACC	72	30
hprt	AGCTTGCTGGTGAAAAGGAC	TTATAGTCAAGGGCATATCC	59	6
rps19	CCTTCCTCAAAAAGTCTGGG	GTTCTCATCGTAGGGAGCAAG	61	10

Primer sequences used in this study and their annealing optimal temperature and time. The mRNA sequences of key genes were obtained from NCBI database. Primers were designed using PRIMER3 software (free on-line access) and checked using Oligo Calculator (free on-line access) and Primer-Blast (NCBI database). Primers sequences are listed in Table 1. hprt and rps19 genes were used as non-regulated reference genes for normalization of target gene expression [22, 23].

ISSN: 1746-6148