|
Gene symbol
|
Forward primer
|
Reverse primer
|
Optimum annealing temp. (°C)
|
Optimum annealing time (sec)
|
|---|
|
pgp
|
GCTTAACACCCGGCTCACAGAC
|
TAAGAAAGCGGCACCAATAGAAAT
|
72
|
10
|
|
bcrp
|
GAGCTCCTTGTGGTTGAGAA
|
AGGTGATGGTCATGAGGAGA
|
72
|
10
|
|
mrp1
|
TTTACTTTTCCCTCGTGCTG
|
TATTCAGGGACCAAAGGTCA
|
72
|
45
|
|
mrp3
|
AGGATGGACCTGATGACAGA
|
AACTTGGGAATCAGGAGACC
|
72
|
30
|
|
hprt
|
AGCTTGCTGGTGAAAAGGAC
|
TTATAGTCAAGGGCATATCC
|
59
|
6
|
|
rps19
|
CCTTCCTCAAAAAGTCTGGG
|
GTTCTCATCGTAGGGAGCAAG
|
61
|
10
|
- Primer sequences used in this study and their annealing optimal temperature and time. The mRNA sequences of key genes were obtained from NCBI database. Primers were designed using PRIMER3 software (free on-line access) and checked using Oligo Calculator (free on-line access) and Primer-Blast (NCBI database). Primers sequences are listed in Table 1. hprt and rps19 genes were used as non-regulated reference genes for normalization of target gene expression [22, 23].
