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Table 1 Used siRNA sequences

From: Expression and role of PGP, BCRP, MRP1 and MRP3 in multidrug resistance of canine mammary cancer cells

Gene GenBank ID First strand Second strand
pgp NM_001003215 CGAACUGUUGUUUCUUUGA[dT][dT] UCAAAGAAACAACAGUUCG[dT][dT]
CUUCCGAACUGUUGUUUCU[dT][dT] AGAAACAACAGUUCGGAAG[dT][dT]
bcrp DQ222459.1 GCCCAGGAGUCAAUGUAAC[dT][dT] GUUACAUUGACUCCUGGGC[dT][dT]
CGAAUAAUACCUGUAGCUA[dT][dT] UAGCUACAGGUAUUAUUCG[dT][dT]
mrp1 NM_001002971 GAAAGAGGCUCCCUGGCAA[dT][dT] UUGCCAGGGAGCCUCUUUC[dT][dT]
GGAGUAUUCAGAAACGGAG[dT][dT] CUCCGUUUCUGAAUACUCC[dT][dT]
mrp3 XM_548204.2 GGCUAUGACGGAGAGCCAA[dT][dT] UUGGCUCUCCGUCAUAGCC[dT][dT]
UGUCUACGCUGCCUUGGGA[dT][dT] UCCCAAGGCAGCGUAGACA[dT][dT]
  1. siRNA sequences used to pgp, bcrp, mrp1 and mrp3 genes silencing in canine mammary cancer cell lines. The canine (Canis lupus familiaris) pgp, bcrp, mrp1 and mrp3 sequences were obtained from Gene Bank with accession numbers: NM_001003215, DQ222459.1, NM_001002971, XM_548204.2, respectively. The siRNA duplexes were designed byhttp://www.sigmaaldrich.com/life-science/custom-oligos/sirna-oligos/sirna-design-service.html. The results were confirmed using two independent algorithms: Dharmacon (OligoWalk) and Ambion.
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BMC Veterinary Research

ISSN: 1746-6148

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