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Table 5 Primer pairs sequences for quantitative real-time PCR 1-4

From: Expression of genes controlling fat deposition in two genetically diverse beef cattle breeds fed high or low silage diets

Gene symbol Full gene name Acc. number 1 Primer pairs (5’-3’)2 Exons spanned Product size (bp)
ACACA Acetyl-CoA carboxylase alpha NM_174224 F: ttc acg tgg cct ggg tag a 40-41 142
    R: ttg tac ctg gat tct cct tca tct t   
CPT1B Carnitine palmitoyltransferase 1B NM_001034349 F: gcg act cca gtg gga cat tc 12-13 114
    R: aaa ggc agg aac tgg aag ca   
CRAT Carnitine O-acetyltransferase NM_001075587 F: ggc cca ccg agc cta cac 12-13 138
    R: atg gca atg gcg tag gag gt   
FABP4 Fatty acid-binding protein 4 NM_174314 F: tgg atg ata aga tgg tgc tgg a 3-4 114
    R: atg gag ttc gat gca aac gtc   
LPL Lipoprotein lipase NM_001075120 F: act gtg gct gag agc gag aac 7-8 98
    R: tct cca ata tcc acc tcc gtg ta   
PPARA Peroxisome proliferator-activated receptor alpha NM_001034036.1 F: agt gcc ttt cag ttg gga tgt c 2-3 125
    R: cgc ggt ttc gga atc ttc ta   
PPARG Peroxisome proliferator-activated receptor gamma NM_181024 F: tgt ctc ata atg cca tca ggt ttg 4-5 66
    R: tct ccg cta aca gct tct cct t   
SCD Stearoyl-CoA desaturase NM_173959 F: cca tca acc ccc gag aga at 5-6 76
    R: aag gtg tgg tgg tag ttg tgg aa   
SREBF1 Sterol regulatory element binding transcription factor 1 NM_001113302 F: agc ctg gca atg tgt gag aag 13-14 115
    R: caa gga gca ggt cac aca gga   
PPIB 3 Peptidylprolyl isomerase B NM_174152 F: tcc gtc ttc ttc ctg ctg ttg 1-2 98
R: cca att cgc agg tca aag tac
RPLP0 4 Ribosomal protein P0 NM_001012682 F: gca ttc ccg ctt cct gg 5-6 109
    R: gcg ctt gta ccc att gat ga   
  1. §Published in [69].
  2. 1Entrez Gene, National Center for Biotechnology Information (NCBI).
  3. 2F: forward primer and R: reverse primer.
  4. 3Housekeeping gene for muscle.
  5. 4Housekeeping gene for subcutaneous adipose tissue.