Expression of genes controlling fat deposition in two genetically diverse beef cattle breeds fed high or low silage diets

Table 5 Primer pairs sequences for quantitative real-time PCR ^1-4

Gene symbol	Full gene name	Acc. number ¹	Primer pairs (5’-3’)²	Exons spanned	Product size (bp)
ACACA	Acetyl-CoA carboxylase alpha	NM_174224	F: ttc acg tgg cct ggg tag a	40-41	142
			R: ttg tac ctg gat tct cct tca tct t
CPT1B	Carnitine palmitoyltransferase 1B	NM_001034349	F: gcg act cca gtg gga cat tc	12-13	114
			R: aaa ggc agg aac tgg aag ca
CRAT	Carnitine O-acetyltransferase	NM_001075587	F: ggc cca ccg agc cta cac	12-13	138
			R: atg gca atg gcg tag gag gt
FABP4	Fatty acid-binding protein 4	NM_174314	F: tgg atg ata aga tgg tgc tgg a	3-4	114
			R: atg gag ttc gat gca aac gtc
LPL	Lipoprotein lipase	NM_001075120	F: act gtg gct gag agc gag aac	7-8	98
			R: tct cca ata tcc acc tcc gtg ta
PPARA	Peroxisome proliferator-activated receptor alpha	NM_001034036.1	F: agt gcc ttt cag ttg gga tgt c	2-3	125
			R: cgc ggt ttc gga atc ttc ta
PPARG	Peroxisome proliferator-activated receptor gamma	NM_181024	F: tgt ctc ata atg cca tca ggt ttg	4-5	66
			R: tct ccg cta aca gct tct cct t
SCD	Stearoyl-CoA desaturase	NM_173959	F: cca tca acc ccc gag aga at	5-6	76
			R: aag gtg tgg tgg tag ttg tgg aa
SREBF1	Sterol regulatory element binding transcription factor 1	NM_001113302	F: agc ctg gca atg tgt gag aag	13-14	115
			R: caa gga gca ggt cac aca gga
PPIB ³	Peptidylprolyl isomerase B	NM_174152	F: tcc gtc ttc ttc ctg ctg ttg	1-2	98
PPIB ³	Peptidylprolyl isomerase B	NM_174152	R: cca att cgc agg tca aag tac	1-2	98
RPLP0 ⁴	Ribosomal protein P0	NM_001012682	F: gca ttc ccg ctt cct gg	5-6	109
			R: gcg ctt gta ccc att gat ga

ISSN: 1746-6148