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Table 2 Summary of observations for selected surface proteins

From: Comparative analysis of the surface exposed proteome of two canine osteosarcoma cell lines and normal canine osteoblasts

Protein name Mass spec qRT-PCR Western blot ICC
Thrombospondin-1     
CnOb D High High N/A
POS ND Low Mid N/A
HMPOS ND Low Low N/A
CD44     
CnOb ND High Low Low
POS D Low High High
HMPOS D Low Mid High
CYR61     
CnOb D High High N/A
POS D Low High N/A
HMPOS D Low Low N/A
Plexin B2     
CnOb ND Low N/A N/A
POS D High N/A N/A
HMPOS D Mid N/A N/A
Notch-2     
CnOb ND Low N/A N/A
POS D Mid N/A N/A
HMPOS D High N/A N/A
  1. A summary of observations for a subset of the surface-exposed proteins identified by mass spectrometry in cultured normal canine osteoblasts (CnOb) and two validated canine osteosarcoma cell lines (POS and HMPOS) and the results of their evaluation via secondary techniques (qRT-PCR, Western blot, and immunocytochemistry [ICC]).
  2. D Detected.
  3. ND Not detected.
  4. High, Mid, Low = relative expression levels observed.