Comparative analysis of the surface exposed proteome of two canine osteosarcoma cell lines and normal canine osteoblasts

Table 1 Subset of mass spectrometry-identified surface proteins of cell lines

Protein name	Gene name	NCBI Ref Sequence	CnOb	POS	HMPOS
Fibronectin	FN1	XP_536059	0.55	0.16	0.14
Annexin 2	ANXA2	NP_001002961	0.15	0.33	0.43
Protein CYR61	CYR61	XP_537091	0.1	0.01	0.48
Vitronectin	VTN	XP_854040	0.1	0.11	0.13
Serpin Peptidase Inhibitor	SERPINH1	NP_001159360	0.17	0.37	0.24
Thrombospondin-1	THBS1	XP_544610	1.1	0	0
Chondroitin sulfate proteoglycan 4	CSPG4	XP_544783	0	2.0	0
CD109 antigen isoform 3	CD109	XP_532205	0	0.21	0
Neuropilin-1 isoform 2	NRP1	XP_535142	0	0.12	0
Glypican-4	GPC4	XP_549265	0	0.11	0
4 F2 cell-surface antigen heavy chain	SLC3A2	XP_540898	0	0.11	0
Cysteine-rich secretory protein LCCL domain-containing 2	CRISPLD2	XP_546797	0	0	0.06
Integrin Beta-1	ITGB1	XP_535143	0.07	0.08	0
Plexin B2	PLXNB2	XP_531689	0	0.77	0.11
CD44 antigen precursor	CD44	NP_001183951	0	0.15	0.07
Ephrin type-A receptor 2 isoform 1	EPHA2	XP_544546	0	0.35	0.06
Inactive tyrosine-protein kinase 7 isoform A	PTK7	XP_538929	0	0.42	0.02
C-type mannose receptor 2	MRC2	XP_003435244	0	0.22	0.01
Delta-sarcoglycan	SGCD	XP_854897	0	0.16	0.01
Notch homolog protein 2 isoform 1	NOTCH2	XP_540266	0	0.08	0.02

A subset of the surface-exposed proteins identified by mass spectrometry in cultured normal canine osteoblasts (CnOb) and two validated canine osteosarcoma cell lines (POS and HMPOS), with their relative spectral counts expressed as a percentage of total spectra.

ISSN: 1746-6148