| Palpebral reflex | Withdrawal reflex | Muscle tension |
---|
 | P1 | P2 | P3A | P3B | P1 | P2 | P3A | P3B | P1 | P2 | P3A | P3B |
Good | 2 | 7 | 6 | 3 | 7 | 10 | 8 | 8 | 6 | 10 | 9 | 9 |
Not Good | 8 | 3 | 4 | 7 | 3 | 0 | 2 | 2 | 4 | 0 | 1 | 1 |
- P1 protocol 1, P2 protocol 2, P3A protocol 3A, and P3B protocol 3B. The reliability was judged by the degree of muscular tension, pedal withdrawal reflex, and palpebral reflex and was afterwards divided into ‘good’ and ‘not good’. The number of animals, which reached at least the palpebral score of 0 were defined as ‘good’ and the animals that never reached a score lower than 1 as ‘not good’. The number of animals reaching a withdrawal score of 0 was judged as ‘good’ and animals never reaching a score lower than 1 were judged as ‘not good’. The number of animals that reached a muscle tension score of maximum 1 was judged as ‘good’ and animals that reached a score of 2 were judged as ‘not good’.