Multiplex PCR-REA applied PCR amplicons before and after digestion with BstE II restrictions enzyme of the Argentine BoHV-5 representative isolates and reference strains. The lanes corresponding to the most recent isolates are marked. Ud: undigested; D: digested; Subt: subtype; Neg.: Control Negative; Ref: reference strain. M: 1 Kb plus DNA size marker.