Isothermal loop-mediated amplification (lamp) for diagnosis of contagious bovine pleuro-pneumonia

BMC Veterinary Research

Table 2 Detection of M. mycoides subsp . mycoides in bronchial lavage fluid of cattle that were experimentally infected by contact with animals that were directly infected 4 weeks before with M. mycoides subsp . mycoides a) strain Afadé [[15]] or b) strain L2 [[15]]

Days before/after contact infection	Viable M. mycoides subsp. *mycoides* / ml	Nested PCR*	LAMP
- 28		-	-
- 7		-	-
+ 13	1.3 × 10⁴	+	+
+ 21	4.0 × 10⁴	+	+
+ 28	3.5 × 10³	+	+
+ 35	2.5 × 10³	+	+
+ 42	7.0 × 10⁷	+	+
+ 49	5.5 × 10⁶	+	+
+ 56	1.8 × 10⁷	+	+
+ 63	7.0 × 10⁸	+	+
+ 70		-	+
+ 77		-	-
+ 84		-	-
+ 92		-	+
+ 98		-	-
+ 105	1.0 × 10²	+	+
+ 112		-	+
+ 119		-	+
+ 126		+	-
+ 134		-	+
+ 141		-	-
+ 147		-	-

ISSN: 1746-6148