|
Gene name
| |
Sequence (5’-3’)
|
Primer
|
Purpose
|
Acc.
|
|---|
| | | |
efficiency (%)
| |
number
|
|---|
|
Profilin2 (Pfn2)
|
F1
|
CCAGCCACTCAATATGTCGT
| |
cloning
|
[GenBank:KC460541]
|
|
R2
|
CAAGAACCTGGGAAACACAC
|
| |
F
|
ATCGCAGCACTTTATTCACC
|
95.18
|
qPCR
| |
|
R
|
GCGTCCTCCTTGTAGAGGTT
|
|
Peroxiredoxin 6 (Prdx6)
|
F
|
TATCGGTGAGACAGGACCAT
| |
cloning
|
[GenBank:KC460539]
|
|
R
|
TGGCGGTTTATTCTAAGTGC
|
| |
F
|
GATGAGATCGACAAGGATGG
|
96.99
|
qPCR
| |
|
R
|
TCTATGACCCTCAGCAGCTC
|
|
Flotillin1 (Flot1)
|
F
|
TCGCTGAAATAGGTCTCTGG
| |
cloning
|
[GenBank:KC460542]
|
|
R
|
ACTCAGCCTTCTTGGTGTTG
|
| |
F
|
ACCAGGACTACCTCCACTCC
|
91.88
|
qPCR
| |
|
R
|
TACTGGGCAGACACCTTCTC
|
|
Calpain small subunit 1 (Capns1)
|
F
|
TTCTCTTCTCACCGCAGAAC
| |
cloning
|
[GenBank:KC460540]
|
|
R
|
GACTTTTCCAGCTCCTCCTC
|
| |
F
|
AACATGGCAACATGGACTTT
|
94.05
|
qPCR
| |
|
R
|
TTGACATCAAGGGAGATGGT
|
|
Beta 2 Tubulin (Tubb2)
|
F
|
CAGCTACTTCGTGGAATGGA
|
94.47
|
qPCR
|
[GenBank:AAD56401]
|
|
R
|
CTGTTGCCGATGAAGGTTAC
|
|
Cold inducible RNA binding protein (Cirbp)
|
F
|
CTCTTCGGAACTCTCAACCA
| |
cloning
|
[GenBank:KC460543]
|
|
R
|
AATACAGCCAGCCAAGTGAC
|
| |
F
|
GTATGGAAACATCGCCAAAG
|
94.17
|
qPCR
| |
|
R
|
CTCGTCGGCATTATCAAACT
|
|
Glutathione S-transferase omega1(Gsto1)
|
F
|
ATTGCGTCTTGGTCATTCAT
| |
cloning
|
[GenBank:KC460544]
|
|
R
|
ACAGGCCATAGTCGTAGTCG
|
| |
F
|
CTGAAACACTTCCTGGATGG
|
95.60
|
qPCR
| |
|
R
|
GCGTGCATGGTTTCTTTAAC
|
|
Ubiquitin (Ubi)
|
F
|
GGCCGCAAAGATGCAGAT
|
96.10
|
qPCR
|
[GenBank:EX735613]
|
|
R
|
CTGGGCTCGACCTCAAGAGT
|
|
Acidic ribosomal protein (Arp)
|
F
|
TGATCCTCCACGACGATGAG
|
95.37
|
qPCR
|
[GenBank:EX741373]
|
|
R
|
CAGGGCCTTGGCGAAGA
|
|
Interleukin 1β (il1β)
|
F
|
GGAGAACACGGACGACCTGA
| |
qPCR
|
[GenBank:AJ535730]
|
| |
R
|
CGCACCATGTCACTGTCCTT
| | | |
- 1Forward primer.
- 2 Reverse primer.
