Figure 4

Relative expression levels of genes (based on the proteome study) in Atlantic cod skin upon V. anguillarum bath challenge. Quantification by real-time PCR. Values are indicated as means ± SEM (N = 5). Transcript levels normalized to reference genes: Ubiquitin (Ubi) and acidic ribosomal protein (Arp). Asterisk (*p < 0.05 or **p < 0.001 or ***p < 0.0001) above the error bars indicate statistically significant difference (Student’s t-test) between infected and control fish, at each time point. Initial samples are depicted as 0h samples. Standard gene abbreviations are used.