Table 3 Real time RT-PCR analysis of peptidase genes in Miamiensis avidus under the different culture conditions
Name of clone | Mean Ctavalue (SDb) | Fold increase in expression (2−ΔΔCt(SD)) | ||
|---|---|---|---|---|
| Â | Cell-free culture | Cell-feeding culture | Cell-free culture | Cell-feeding culture |
MaPro 1 | 28.48(0.51) | 22.67(0.32) | 1.05(0.40) | 1.01(0.22) |
MaPro 2** | 25.38(0.24) | 21.25(0.12) | 1.01(0.16) | 0.31(0.03) |
MaPro 3 | 27.43(0.34) | 22.11(0.10) | 1.02(0.23) | 0.71(0.05) |
MaPro 4** | 34.96(0.28) | 21.95(0.24) | 1.02(0.20) | 147.58(24.89) |
MaPro 5 | 33.19(0.44) | 29.71(0.63) | 1.03(0.30) | 0.21(0.08) |
MaPro 6** | 23.93(0.36) | 20.01(0.19) | 1.02(0.27) | 0.27(0.04) |
MaPro 7* | 33.61(0.31) | 25.72(0.11) | 1.01(0.22) | 4.19(0.33) |
MaPro 8** | 26.94(0.21) | 24.11(0.31) | 1.01(0.14) | 0.13(0.03) |
MaPro 9* | 29.33(0.58) | 26.79(0.58) | 1.05(0.42) | 0.11(0.04) |
MaPro 10 | 32.67(1.44) | 26.44(0.29) | 1.29(0.86) | 1.34(0.27) |
MaPro 11 | 30.26(1.30) | 25.09(0.25) | 1.24(0.79) | 0.64(0.11) |
MaPro 12** | 25.57(0.08) | 22.90(0.29) | 1.00(0.06) | 0.11(0.02) |
MaPro 13* | 34.37(0.34) | 31.15(1.30) | 1.02(0.22) | 0.21(0.16) |
MaPro 14** | 32.51(0.41) | 19.37(0.01) | 1.03(0.27) | 159.78(1.40) |
MaPro 15 | N/Ac | 31.12(0.76) | Â | Â |
MaPro 16 | N/A | 31.52(0.57) | Â | Â |
MaPro 17* | 34.68(0.77) | 26.87(0.32) | 1.10(0.54) | 4.04(0.84) |
β-tubulin | 23.37(0.45) | 17.55(0.22) |  |  |