Transcriptome and proteome analysis of tyrosine kinase inhibitor treated canine mast cell tumour cells identifies potentially kit signaling-dependent genes

Table 2 Down- or up-regulated proteins after 72 hours masitinib treatment

Spot.	Identified protein	AV	t test	accession no. in NCBI	MW [Da]²	pI^§	MOWSE score	assigned peptides	sequ. cov. [%]
Down-regulated proteins
1	TARDBP43	−2.62	0.019	gi\|6678271 (Ho)	45053	5.85	53	1*	4*
6	EIF4A	−1.66	0.005	gi\|73966207 (Ca)	44692	5.40	89	15	41
7	TCP1A	−1.63	0.019	gi\|73945755 (Ca)	60943	5.71	80	9	17
2	EIF3	−1.33	0.012	gi\|73974369 (Ca)	29504	7.78	92	8	21
8	PPA1	−1.25	0.021	gi\|73953384 (Ca)	33372	5.80	34	1*	2*
9	CCT2	−1.25	0.042	gi\|73968673 (Ca)	57737	6.01	80	8	17
3	ACTR2	−1.12	0.019	gi\|73969816 (Ca)	36488	8.21	46	1*	4*
10	PSMD6	−1.06	0.040	gi\|73981961 (Ca)	99123	9.57	72	10	12
Up-regulated proteins
11	HSP90B1	+1.18	0.048	gi\|50979116 (Ca)	22925	6.23	74	5	24
11	GSTT3	+1.20	0.0077	gi\|73995693 (Ca)	27793	6.67	98	8	33
12	YWHAZ.	+1.22	0.019	gi\|73974186 (Ca)	26438	5.00	156	17	52
13	PDIA3	+1.26	0.012	gi\|74000349 (Ca)	76474	8.95	161	21	30
14	CORO1A	+1.34	0.017	gi\|73958506 (Ca)	41955	7.10	52	1*	2*
15	TTC38	+1.34	0.016	gi\|73968897 (Ca)	52886	5.67	79	6	12
16	OXCT1	+1.43	0.028	gi\|73954281 (Ca)	65275	7.92	90	10	18
17	SEMG1	+1.45	0.017	gi\|134093092 (Go)	52065	8.96	83	8	19
18	UROD	+1.45	0.016	gi\|73977144 (Ca)	35096	5.50	105	9	29
19	ANXA6	+1.50	0.031	gi\|73953627 (Ca)	76241	5.47	207	23	32
20	UCHL3	+1.51	0.027	gi\|158749588 (Ra)	26278	5.01	74	5	23
21	SELENBP1	+1.65	0.0011	gi\|73981582 (Ca)	67142	7.59	89	9	20
22	IRX6	+1.74	0.0018	gi\|121484129 (Pa)	24682	8.99	74	5	24
5	CAPG	+1.98	0.0036	gi\|73980918 (Ca)	39088	6.04	85	6	24
4	ANXA1	+2.58	0.007	gi\|73946797 (Ca)	38887	5.84	91	12	31

^§The listed molecular weights and pI values correspond to the listed accession numbers, which sometimes belong to species other than Canis familiaris (Ca).
*These proteins were identified by unique peptide sequences determined by MALDI-TOF MS/MS experiments.
Abbreviations: EIF4A: eukaryotic translation initiation factor 4A; TCP-1-alpha: T-complex protein 1, alpha subunit; EIF3: eukaryotic translation initiation factor 3; ACTR2: actin-related protein 2; EIF3: eukaryotic translation initiation factor 3; HSP-ß1: heat shock protein beta-1; PDI: protein disulfide isomerase-associated 3 precursor; CORO-1A: coronin-1A; UPD: Uroporphyrinogen decarboxylase; ANXA6: annexin A6; MCP: Macrophage capping protein; ANXA1: annexin A1; Ca: Canis familiaris; Ho: Homo sapiens; Pan: Pan troglodytes; Ra: Rattus; Go: Gorilla.

ISSN: 1746-6148