Transcriptome and proteome analysis of tyrosine kinase inhibitor treated canine mast cell tumour cells identifies potentially kit signaling-dependent genes

Table 1 Down- or up-regulated proteins after 24 hours masitinib treatment

Spot	Identified protein	AV	p-value	accession no. in NCBI	MW [Da]^§	pI^§	MOWSE score	assigned peptides	sequ. cov. [%]
Down-regulated proteins
1	TARDBP	−1.52	0.012	gi\|6678271 (Ho)	45053	5.85	53	1*	4*
2	EIF3	−1.30	0.014	gi\|73974369 (Ca)	29504	7.78	92	8	21
3	ACTR2	−1.09	0.0054	gi\|73969816 (Ca)	36488	8.21	46	1*	4*
Up-regulated proteins
4	ANXA1	+1.66	0.0087	gi\|73946797 (Ca)	38887	5.84	91	12	31
5	CAPG	+1.66	0.0039	gi\|73980918 (Ca)	39088	6.04	85	6	24

^§The listed molecular weights and pI values correspond to the listed accession numbers, which sometimes belong to species other than Canis familiaris (Ca.).
*These proteins were identified by unique peptide sequences determined by MALDI-TOF MS/MS experiments.
Abbreviations: EIF3: eukaryotic translation initiation factor 3; ACTR2: actin-related protein 2; ANXA1: annexin A1; MCP: Macrophage capping protein; Ca: Canis familiaris; Ho: Homo sapiens.

ISSN: 1746-6148