MUC1/CA 15–3 expression in canine mammary cancer cell lines and the human MCF7 cell line. A. Pictures of MCF7 human mammary carcinoma cells and canine mammary cancer cell lines: P114, CMT-U27, CMT-U309, CMT-W1, CMT-W1M, CMT-W2 and CMT-W2M obtained with an Olympus BX60 microscope (at the magnification of 200x). The MUC1 antigen is reflected as a brown colour. B. The graph of the mean MUC1 expression (and SE) in the examined cell lines. Ten pictures in each slide were analysed. The brown colour intensity was counted by a computer-assisted image analyser (Olympus Microimage™ Image Analysis, software version 4.0 for Windows, USA). The statistical analysis was performed using Prism version 5.00 software (GraphPad Software, USA). The ANOVA and Dunett`s Multiple Comparison post-hoc test were applied to analyse the MUC1 expression in the examined cell lines (the MCF7 cell line was regarded as a control); p < 0.001 was regarded as highly significant and marked as ***, whereas p < 0.05 was regarded as significant and marked as *.